Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F15%3A00085534" target="_blank" >RIV/00216224:14740/15:00085534 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/15:00446295
Result on the web
<a href="http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14" target="_blank" >http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-015-1436-5" target="_blank" >10.1007/s00706-015-1436-5</a>
Alternative languages
Result language
angličtina
Original language name
Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes
Original language description
In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin-alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and itsapplicability for off-line monitoring of DNA amplification is demonstrated.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Monatshefte fur Chemie
ISSN
0026-9247
e-ISSN
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Volume of the periodical
146
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
849-855
UT code for WoS article
000353219700015
EID of the result in the Scopus database
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