Stable gene replacement in barley by targeted double-strand break induction
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F16%3A00093884" target="_blank" >RIV/00216224:14740/16:00093884 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv537" target="_blank" >https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv537</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jxb/erv537" target="_blank" >10.1093/jxb/erv537</a>
Alternative languages
Result language
angličtina
Original language name
Stable gene replacement in barley by targeted double-strand break induction
Original language description
Using double-strand break induction in the crop plant barley, standard transformation technology allows gene replacement to occur at frequencies suitable for routine application.Gene targeting is becoming an important tool for precision genome engineering in plants. During gene replacement, a variant of gene targeting, transformed DNA integrates into the genome by homologous recombination (HR) to replace resident sequences. We have analysed gene targeting in barley (Hordeum vulgare) using a model system based on double-strand break (DSB) induction by the meganuclease I-SceI and a transgenic, artificial target locus. In the plants we obtained, the donor construct was inserted at the target locus by homology-directed DNA integration in at least two transformants obtained in a single experiment and was stably inherited as a single Mendelian trait. Both events were produced by one-sided integration.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.20.0189" target="_blank" >EE2.3.20.0189: Development of excellence in evolutionary cytogenomics, epigenetics and cell signalling</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Experimental Botany
ISSN
0022-0957
e-ISSN
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Volume of the periodical
67
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
1433-1445
UT code for WoS article
000371928000021
EID of the result in the Scopus database
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