MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00382526" target="_blank" >RIV/61389030:_____/12:00382526 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkr1272" target="_blank" >http://dx.doi.org/10.1093/nar/gkr1272</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkr1272" target="_blank" >10.1093/nar/gkr1272</a>

Result language

angličtina

Original language name

MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens

Original language description

The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DNA DSBs, the MRN complex. Loss of function of PpMRE11 or PpRAD50 strongly and specifically inhibited gene targeting, whilst rates of untargeted transgene integration were relatively unaffected. In contrast, disruption of the PpNBS1 gene retained the wild-type capacity to integrate transforming DNA efficiently at homologous loci. Analysis of the kinetics of DNA-DSBrepair in wild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-induced fragmentation of genomic DNA was repaired at approximately equal rates in each genotype, although both the Ppmre11 and Pp

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

3496-3510

UT code for WoS article

000303333500024

EID of the result in the Scopus database

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