High-frequency, precise modification of the tomato genome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00456698" target="_blank" >RIV/68081707:_____/15:00456698 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s13059-015-0796-9" target="_blank" >http://dx.doi.org/10.1186/s13059-015-0796-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13059-015-0796-9" target="_blank" >10.1186/s13059-015-0796-9</a>
Alternative languages
Result language
angličtina
Original language name
High-frequency, precise modification of the tomato genome
Original language description
Background: The use of homologous recombination to precisely modify plant genomes has been challenging, due to the lack of efficient methods for delivering DNA repair templates to plant cells. Even with the advent of sequence-specific nucleases, which stimulate homologous recombination at predefined genomic sites by creating targeted DNA double-strand breaks, there are only a handful of studies that report precise editing of endogenous genes in crop plants. More efficient methods are needed to modify plant genomes through homologous recombination, ideally without randomly integrating foreign DNA. Results: Here, we use geminivirus replicons to create heritable modifications to the tomato genome at frequencies tenfold higher than traditional methods of DNA delivery (i.e., Agrobacterium). A strong promoter was inserted upstream of a gene controlling anthocyanin biosynthesis, resulting in overexpression and ectopic accumulation of pigments in tomato tissues. More than two-thirds of the ins
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LH14002" target="_blank" >LH14002: Functional Genomics of Dioecious Plants</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genome Biology
ISSN
1465-6906
e-ISSN
—
Volume of the periodical
16
Issue of the periodical within the volume
NOV 6 2015
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
—
UT code for WoS article
000365318200001
EID of the result in the Scopus database
—