Sensitive determination of malondialdehyde in exhaled breath condensate and biological fluids by capillary electrophoresis with laser induced fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00095240" target="_blank" >RIV/00216224:14740/17:00095240 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0039914017303570?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914017303570?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2017.03.061" target="_blank" >10.1016/j.talanta.2017.03.061</a>

Result language

angličtina

Original language name

Sensitive determination of malondialdehyde in exhaled breath condensate and biological fluids by capillary electrophoresis with laser induced fluorescence detection

Original language description

In this work, a sensitive capillary electrophoresis method with laser induced fluorescence detection for determination of malondialdehyde in various biological fluids was developed. Malondialdehyde reacts with thiobarbituric acid under optimized conditions of pH=2, reaction time of 60 min and temperature of 90 degrees C, yielding an adduct that can be separated in a 50 mM sodium borate background electrolyte at pH 9. The separation of the formed adduct was accomplished in less than 6 min with limit of detection of 1.1 nM due to the use of 532 nm laser module, exactly matching the maximum excitation wavelength of the formed adduct. The developed method offers unprecedented sensitivity and was for the first time used for analysis of malondialdehyde in exhaled breath condensate. The method proved to be also applicable to other samples of biological fluids, such as blood plasma and saliva.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

169

Issue of the periodical within the volume

JUL

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

85-90

UT code for WoS article

000401206700013

EID of the result in the Scopus database

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