Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00106734" target="_blank" >RIV/00216224:14740/18:00106734 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.matdes.2018.05.002" target="_blank" >http://dx.doi.org/10.1016/j.matdes.2018.05.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.matdes.2018.05.002" target="_blank" >10.1016/j.matdes.2018.05.002</a>
Alternative languages
Result language
angličtina
Original language name
Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization
Original language description
Herein COOH/anhydride functionalized biodegradable nanofibers were prepared using atmospheric pressure plasma copolymerization of maleic anhydride (MA) and C2H2. The XPS and ATR-FTIR analyses revealed a high concentration of carboxyl and anhydride groups that were used for grafting of gentamycin (GM) onto the surface of PCL nanofibers. It was shown that GM can be immobilized either with or without dicyclohexyl carbodiimde (DCC). The immobilization without DCC led to ionic bonding (PCL-MA-GMi), whereas the immobilization using DCC activation resulted in covalent bonding (PCL-MA-GMc). The investigation of IAR2 epithelial cell adhesion and proliferation revealed that the GM-loaded nanofibers were biocompatible. The nanofibers without immobilized GM did not show any noticeable antibacterial activity against E. coli bacteria with different resistance to gentamicin. In contrast, the size of inhibition zone around the PCL-MA-GMi and PCL-MA-GMc samples reached 27 mm, hereby indicating a strong antibacterial effect against all types of E. coli bacteria. The GM-loaded nanofibers also demonstrated a pronounced antibacterial effect after immersion in phosphate buffered saline at 37 degrees C for 24 h. Thus the results demonstrated that the proposed strategy for the preparation of antibacterial biocompatible nanofibers with relatively long-termantibacterial protection has a great potential for future application for wound healing. (C) 2018 Elsevier Ltd. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MATERIALS & DESIGN
ISSN
0264-1275
e-ISSN
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Volume of the periodical
153
Issue of the periodical within the volume
SEP
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
60-70
UT code for WoS article
000436433600007
EID of the result in the Scopus database
2-s2.0-85046805335