Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F18%3APU128978" target="_blank" >RIV/00216305:26620/18:PU128978 - isvavai.cz</a>

Result on the web

<a href="https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0264127518303794" target="_blank" >https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0264127518303794</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matdes.2018.05.002" target="_blank" >10.1016/j.matdes.2018.05.002</a>

Result language

angličtina

Original language name

Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

Original language description

Herein COOH/anhydride functionalized biodegradable nanofibers were prepared using atmospheric pressure plasma copolymerization of maleic anhydride (MA) and C2H2. The XPS and ATR-FTIR analyses revealed a high concentration of carboxyl and anhydride groups that were used for grafting of gentamycin (GM) onto the surface of PCL nanofibers. It was shown that GM can be immobilized either with or without dicyclohexyl carbodiimde (DCC). The immobilization without DCC led to ionic bonding (PCL-MA-GMi), whereas the immobilization using DCC activation resulted in covalent bonding (PCL-MA-GMc). The investigation of IAR2 epithelial cell adhesion and proliferation revealed that the GM-loaded nanofibers were biocompatible. The nanofibers without immobilized GM did not show any noticeable antibacterial activity against E. coli bacteria with different resistance to gentamicin. In contrast, the size of inhibition zone around the PCL-MA-GMi and PCL-MA-GMc samples reached 27 mm, hereby indicating a strong antibacterial effect against all types of E. coli bacteria. The GM-loaded nanofibers also demonstrated a pronounced antibacterial effect after immersion in phosphate buffered saline at 37 °C for 24 h. Thus the results demonstrated that the proposed strategy for the preparation of antibacterial biocompatible nanofibers with relatively long-term antibacterial protection has a great potential for future application for wound healing.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10302 - Condensed matter physics (including formerly solid state physics, supercond.)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials and design

ISSN

0261-3069

e-ISSN

—

Volume of the periodical

153

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

60-70

UT code for WoS article

000436433600007

EID of the result in the Scopus database

2-s2.0-85046805335