In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00108251" target="_blank" >RIV/00216224:14740/19:00108251 - isvavai.cz</a>
Result on the web
<a href="https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71" target="_blank" >https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cpnc.71" target="_blank" >10.1002/cpnc.71</a>

Result language
angličtina
Original language name
In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Original language description
In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed instructions are described for setting up an in-cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step-by-step protocols for both the preparation of an in-cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in-cell NMR experiments are discussed.
Czech name
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Czech description
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Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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