Memory CD4(+) T cells are generated in the human fetal intestine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113268" target="_blank" >RIV/00216224:14740/19:00113268 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/s41590-018-0294-9" target="_blank" >http://dx.doi.org/10.1038/s41590-018-0294-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41590-018-0294-9" target="_blank" >10.1038/s41590-018-0294-9</a>
Alternative languages
Result language
angličtina
Original language name
Memory CD4(+) T cells are generated in the human fetal intestine
Original language description
The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO(+) T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4(+) T cell compartment in the human fetal intestine. We identified 22 CD4(+) T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4(+) T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-gamma and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4(+) T cells. Imaging mass cytometry indicated that memory-like CD4(+) T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4(+) T cells in the human fetal intestine that is consistent with exposure to foreign antigens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature immunology
ISSN
1529-2908
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
301-314
UT code for WoS article
000458893600015
EID of the result in the Scopus database
2-s2.0-85060343432