CRK5 Protein Kinase Contributes to the Progression of Embryogenesis of Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00113437" target="_blank" >RIV/00216224:14740/19:00113437 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1422-0067/20/24/6120" target="_blank" >https://www.mdpi.com/1422-0067/20/24/6120</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms20246120" target="_blank" >10.3390/ijms20246120</a>

Result language

angličtina

Original language name

CRK5 Protein Kinase Contributes to the Progression of Embryogenesis of Arabidopsis thaliana

Original language description

The fine tuning of hormone (e.g., auxin and gibberellin) levels and hormone signaling is required for maintaining normal embryogenesis. Embryo polarity, for example, is ensured by the directional movement of auxin that is controlled by various types of auxin transporters. Here, we present pieces of evidence for the auxin-gibberellic acid (GA) hormonal crosstalk during embryo development and the regulatory role of the Arabidopsis thaliana Calcium-Dependent Protein Kinase-Related Kinase 5 (AtCRK5) in this regard. It is pointed out that the embryogenesis of the Atcrk5-1 mutant is delayed in comparison to the wild type. This delay is accompanied with a decrease in the levels of GA and auxin, as well as the abundance of the polar auxin transport (PAT) proteins PIN1, PIN4, and PIN7 in the mutant embryos. We have previously showed that AtCRK5 can regulate the PIN2 and PIN3 proteins either directly by phosphorylation or indirectly affecting the GA level during the root gravitropic and hypocotyl hook bending responses. In this manuscript, we provide evidence that the AtCRK5 protein kinase can in vitro phosphorylate the hydrophilic loops of additional PIN proteins that are important for embryogenesis. We propose that AtCRK5 can govern embryo development in Arabidopsis through the fine tuning of auxin-GA level and the accumulation of certain polar auxin transport proteins.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1661-6596

Volume of the periodical

20

Issue of the periodical within the volume

24

Country of publishing house

CH - SWITZERLAND

Number of pages

23

Pages from-to

1-23

UT code for WoS article

000506840100015

EID of the result in the Scopus database

2-s2.0-85076307723