Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F21%3A00124251" target="_blank" >RIV/00216224:14740/21:00124251 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2306-7381/8/3/48" target="_blank" >https://www.mdpi.com/2306-7381/8/3/48</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/vetsci8030048" target="_blank" >10.3390/vetsci8030048</a>
Alternative languages
Result language
angličtina
Original language name
Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes
Original language description
The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 x 10(6) CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 x 10(4) to 6.67 x 10(4) CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Polish Journal of Veterinary Sciences
ISSN
2306-7381
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
48
UT code for WoS article
000634239500001
EID of the result in the Scopus database
2-s2.0-85103343525