Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F23%3A00134306" target="_blank" >RIV/00216224:14740/23:00134306 - isvavai.cz</a>
Result on the web
<a href="https://www.imp.ac.at/research/cryo-em-symposium" target="_blank" >https://www.imp.ac.at/research/cryo-em-symposium</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM
Original language description
Tick-borne encephalitis (TBE), caused by the tick-borne encephalitis virus (TBEV), is a severe disease that can lead to fatal central nervous system inflammation. Despite the availability of vaccines, vaccination rates in the most affected areas, such as the Czech Republic, are low. With TBE cases on the rise, the need for targeted treatments is growing. Antibodies have shown promise in a mouse model, where intravenous administration of TBEV-specific antibodies served as a treatment. However, our understanding of the molecular mechanisms behind TBEV neutralisation remains limited.To address this gap, we investigated the interactions between the TBEV Neudörfl strain and two neutralising mouse monoclonal antibodies, IC3 and A4, which bind to distinct domains of the TBEV envelope protein. We purified TBEV from infected tissue culture cells, mixed it with Fab fragments derived from the neutralising antibodies, and vitrified the samples on grids for cryo-electron microscopy. Using single-particle analysis, we solved the structures of the TBEV-Fab complexes from the collected micrographs.By deciphering the molecular basis of TBEV neutralisation by antibodies, we aim to gain insights into the significance of different epitopes on the viral surface. This knowledge may pave the way for the tailored design of therapeutic antibodies or more specific vaccines in the future.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/GX19-25982X" target="_blank" >GX19-25982X: Structural study of enterovirus replication in situ</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů