Genome editing in almond using hairy root transformation system
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F24%3A00137955" target="_blank" >RIV/00216224:14740/24:00137955 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s11240-024-02935-x" target="_blank" >https://doi.org/10.1007/s11240-024-02935-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-024-02935-x" target="_blank" >10.1007/s11240-024-02935-x</a>
Alternative languages
Result language
angličtina
Original language name
Genome editing in almond using hairy root transformation system
Original language description
Woody plant species are often challenging to transform, which complicates gene function studies using molecular biology techniques. To develop an effective gene editing tool for almond (Prunus dulcis, syn. Prunus amygdalus), we established a new method for inducing hairy roots in almond seedlings based on the injection of agrobacterial culture. We generated composite plants comprising wild-type shoots and transgenic hairy roots. This approach was also successful in almond x peach hybrids. The hairy root transformation system was used alongside Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 gene editing to target two transcription factor genes (ETHYLENE RESPONSE FACTOR 74 and GIBBERELLIC ACID INSENSITIVE). We successfully generated hairy roots with knockouts of the target genes and evaluated the efficiency of guide RNAs for gene editing in almond. Our transgenic root method could be a valuable tool for routine gene function studies in almond.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell, Tissue and Organ Culture (PCTOC)
ISSN
0167-6857
e-ISSN
1573-5044
Volume of the periodical
159
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
1-11
UT code for WoS article
001374123900002
EID of the result in the Scopus database
2-s2.0-85211431070