HAIRY ROOTS AS A POWERFUL TOOL TO STUDY HORMONAL AND ABIOTIC STRESS RESPONSE

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143963" target="_blank" >RIV/00216224:14740/25:00143963 - isvavai.cz</a>

Result on the web

<a href="https://www.recrop-cost.com/NewsAndActivities/ReadMore/8dd16317-8fc8-488e-dca1-08dddc48cd1e" target="_blank" >https://www.recrop-cost.com/NewsAndActivities/ReadMore/8dd16317-8fc8-488e-dca1-08dddc48cd1e</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HAIRY ROOTS AS A POWERFUL TOOL TO STUDY HORMONAL AND ABIOTIC STRESS RESPONSE

Original language description

Hairy root cultures are a versatile system with many applications, including the production of recombinant proteins and valuable secondary metabolites, as well as the functional characterisation of genes. We have developed efficient transformation protocols for the spring cultivars of Brassica napus and Brassica rapa using hairy roots. We have generated various reporter lines, including new fluorescent reporters that monitor the response of the plant hormones auxin and cytokinin to abiotic stress stimuli. Furthermore, we have developed a pipeline for gene editing and hairy root regeneration in B. napus, with the aim of creating transgene-free, gene-edited plants. Since oilseed rape is an allotetraploid species (AACC, 2n=38), mutating homoeologs from both subgenomes is necessary to overcome functional genetic redundancy. Using optimised CRISPR/Cas9 vectors, we generated multiple mutant lines in four B. napus TRYPTOPHAN AMINOTRANSFERASE (BnaTAA1) auxin biosynthetic genes. In conclusion, we have initiated a functional analysis of BnaTAA1 and a study of the auxin biosynthesis pathway in oilseed rape using hairy root cultures. We offer transformation services for selected oilseed rape cultivars to generate CRISPR-edited plants and reporter lines. Funding sources: GA23-06140S by the Czech Science Foundation and TANGENC (TowArds Next GENeration Crops) CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius by MEYS.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: TowArds Next GENeration Crops (TANGENC)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů