CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143990" target="_blank" >RIV/00216224:14740/25:00143990 - isvavai.cz</a>
Result on the web
<a href="https://www.potravinyav21.cz/download/1826?utm_source=chatgpt.com" target="_blank" >https://www.potravinyav21.cz/download/1826?utm_source=chatgpt.com</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
CRISPR-BASED GENOME EDITING IN BRASSICACEAE USING TISSUE CULTURE SYSTEMS
Original language description
Hairy root cultures represent a versatile system applicable to a wide range of studies, including the functional characterization of genes and their regulatory elements. In our laboratory, we have established a CRISPR-based genome editing protocol for oilseed rape (Brassica napus) using hairy roots. This transformation system offers a straightforward and rapid approach to evaluate the functionality and efficiency of diverse CRISPR editing constructs. Using an optimized regeneration protocol, we are able to produce transgene-free, genome edited plant lines. Our future objectives involve the application of CRISPR technologies for base editing, prime editing, and transcriptional regulation within the Brassicaceae family using hairy root cultures.
Czech name
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Czech description
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Project
<a href="/en/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: TowArds Next GENeration Crops (TANGENC)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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