Multimodal Nanoscale Characterization of Biological Samples Using Atomic Force Microscopy and Complementary Techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00142553" target="_blank" >RIV/00216224:14740/25:00142553 - isvavai.cz</a>

Result on the web

<a href="https://www.sci.muni.cz/czesev/czesev-2025" target="_blank" >https://www.sci.muni.cz/czesev/czesev-2025</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Multimodal Nanoscale Characterization of Biological Samples Using Atomic Force Microscopy and Complementary Techniques

Original language description

Atomic Force Microscopy (AFM) enables high-resolution imaging, nanomechanical mapping, and single-molecule force spectroscopy under near-physiological conditions. Our facility specializes in AFM applications to diverse biological samples, enhanced by correlative techniques including Raman and fluorescence microscopy, polarization microscopy, and microelectrode arrays (MEA). This multimodal approach allows simultaneous acquisition of mechanical, structural, and chemical data, enhancing analysis of complex biological systems. We apply these methods across a range of studies, including stiffness mapping of living cells, mechanobiology of cardiac and hepatic tissues, and chemical imaging of amyloid deposits in Alzheimer’s disease. Key research highlights include elucidation of protein fibrillization, assessment of nanoparticle effects on cell functions, and investigation of liver fibrosis dynamics. This platform is also highly suitable for structural and mechanical studies of liposomes and extracellular vesicles. By fostering interdisciplinary collaboration and offering open-access services to academic and industry users, we provide comprehensive workflows, training, and data processing support. Our integrated strategy exemplifies the power of AFM-based multimodal microscopy to probe the interplay between structure, mechanics, and chemistry at multiple biological scales. The Core Facility Nanobiotechnology at CEITEC Masaryk University plays a vital role in advancing bioscience research by providing access to state-of-the-art instrumentation and expert consultation, making it an excellent partner for the characterization of extracellular vesicles and complex biological samples.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů