PHD-HD PROTEINS: AN ENIGMATIC PLANT-SPECIFIC TRANSCRIPTION FACTOR FAMILY
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F25%3A00143779" target="_blank" >RIV/00216224:14740/25:00143779 - isvavai.cz</a>
Result on the web
<a href="https://csebr.cz/plant-biology-cs-2025/" target="_blank" >https://csebr.cz/plant-biology-cs-2025/</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
PHD-HD PROTEINS: AN ENIGMATIC PLANT-SPECIFIC TRANSCRIPTION FACTOR FAMILY
Original language description
Transcription factors (TFs) are key regulators of gene expression and plant development. The plant-specific PHD-HD TF family, defined by a plant homeodomain (PHD) and homeodomain (HD) architecture, is represented in Arabidopsis thaliana by only two members: HAT3.1 and PRHA. Despite their close phylogenetic relationship, these proteins diverge substantially in molecular features, subnuclear organization, and developmental roles. HAT3.1 forms distinct nuclear condensates that partially colocalize with chromocenters. TurboID-based proximity labeling identified methyl-CpG-binding domain proteins (MBD1/2/4) as top interactors of HAT3.1; these co-localize with HAT3.1 speckles, suggesting formation of functional nuclear complexes. Furthermore, a histone peptide array revealed that the PHD domain of HAT3.1 specifically recognizes H4K20me3, a histone modification with largely unexplored roles in plants. In contrast, PRHA displays diffuse nuclear localization, and its PHD domain lacks histone binding specificity. Promoter-reporter analyses demonstrate that HAT3.1 is expressed throughout the meristematic zone of the root apical meristem, while PRHA expression is confined to initials and pericycle cells. Both genes are upregulated during callus induction and shoot regeneration, indicating a role in cellular reprogramming. Notably, prha mutants exhibit accelerated callus formation and enhanced regeneration capacity, suggesting a role in repressing cell proliferation. In summary, our data revealed a pronounced functional divergence within the PHD-HD TF family and pointed to their distinct contributions to chromatin-associated regulation and developmental reprogramming in plants.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů