Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F04%3A00001456" target="_blank" >RIV/00216275:25310/04:00001456 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/04:00088382 RIV/00216275:25310/04:00001455
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Utilization of newly developed immobilized enzyme reactors for preparation and study of immunoglobulin G fragments
Original language description
The newly developed immobilized enzyme reactors (IMERs) with proteolytic enzymes chymotrypsin, trypsin or papain were used for specific fragmentation of high molecular-mass and heterogeneous glycoproteins immunoglobulin G (IgG) and crystallizable fragment of IgG (Fc). The efficiency of splitting or digestion were controlled by RP-HPLC. The specificity of digestion by trypsin reactor was controlled by MS. IMERs (trypsin immobilized on magnetic microparticles focused in a channel of magnetically active microfluidic device) was used for digestion of the whole IgG molecule. The sufficient conditions for IgG digestion in microfluidic device (flow rate, ratio S:E, pH, temperature) were optimized. It was confirmed that the combination of IMERs with microfluidic device enables efficient digestion of highly heterogeneous glycoproteins such as IgG in extremely short time and minimal reaction volume.
Czech name
Využití nově vyvinutých imobilizovaných enzymových reaktorů pro přípravu a studium fragmentů imunoglobulinu G
Czech description
Využití nově vyvinutých imobilizovaných enzymových reaktorů pro přípravu a studium fragmentů imunoglobulinu G

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GA203%2F02%2F0023" target="_blank" >GA203/02/0023: Development of new procedures for highly reliable analytical control of technologies,materials,food &environment & for med. diagnostic using modern instrumentat</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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