Chip-based bioaffinity reactor with immobilized anhydrotrypsin for isolation of specific tryptic peptides of human neurotensin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002851" target="_blank" >RIV/00216275:25310/05:00002851 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Chip-based bioaffinity reactor with immobilized anhydrotrypsin for isolation of specific tryptic peptides of human neurotensin

Original language description

One of the modern trends in biochemistry is focused on the utilization of microfluidic device for protein analysis due to many advantages (low sample consumption, highthroughput analysis etc.). Functional spherical nanoparticles with controlled tendencyto aggregate and controlled level of nonspecific sorption could be focused in channel of magnetically active microfluidic device for isolation or enzyme modification of target protein directly in a channel of microfluidic device. Biospecific carrier prepared by immobilization of anhydrotrypsin on magnetic nanoparticles was used for purification, isolation and preconcentration of specific peptides from complex mixture of tryptic peptides in one step. IMER (immobilized magnetic enzyme reactor) with trypsin in tandem with chip-based IMAR (immobilized magnetic affinity reactor) with anhydrotrypsin was used for simplification of peptide mapping including the digestion of protein coupling with purification and preconcentration of peptides for

Czech name

Bioafinitní reaktor založený na imobilizovaném anhydrotrypsinu, izolace neurotensinových peptidů

Czech description

Bioafinitní reaktor založený na imobilizovaném anhydrotrypsinu, izolace neurotensinových peptidů.

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Mezinárodní sjezd chemické společnosti

ISBN

1336-7242

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

267-268

Publisher name

Slovenská Chemická spoločnost

Place of publication

Bratislava, Slovensko

Event location

Tatranské Matliare, Slovensko

Event date

Sep 4, 2005

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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