Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002852" target="_blank" >RIV/00216275:25310/05:00002852 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Chip-based magnetic separation technique with immobilized trypsin and anhydrotrypsin for isolation of specific peptides of neurotensin
Original language description
The isolation of proteins and peptides in the area of biosciences and biotechnology is now the subject of interest. Magnetic separation techniques have several advantages in comparison with standard separation procedures. Functional spherical nanoparticles with controlled tendency to aggregate and controlled level of nonspecific sorption could be focused in channel of magnetically active microfluidic device for isolation or enzyme modification of target protein directly in a microchannel. Biospecific ligand anhydrotrypsin enables highly specific trapping of peptides which correspond to the products generated by the action of various trypsin-like proteases and binds, in slightly acidic conditions, only specific peptides containing arginine and lysine residues at their C-termini [1]. Anhydrotrypsin is catalytically inactive derivative of bovine trypsin in which serine residue (Ser-195) is first modified with phenylmethanesulfonyl fluoride (PMSF), an active site-directed reagent, and then
Czech name
Separace založená na biočipové technologii, specifická izolace tryptických peptidů pomocí anhydrotrypsinu.
Czech description
Separace založená na biočipové technologii, specifická izolace tryptických peptidů pomocí anhydrotrypsinu.

Project
<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
11th International Symposium on Separation Science (ISSS 2005)
ISBN
80-7194-771-7
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
234
Publisher name
Univerzita Pardubice
Place of publication
pardubice
Event location
Pardubice
Event date
Sep 12, 2005
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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