Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F05%3A00002848" target="_blank" >RIV/00216275:25310/05:00002848 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Kinetics of Hydrolysis of Acetylthiocholine and Acetylcholine by Cholinesterases.

Original language description

Kinetics of hydrolysis of acetylthiocholine (ATCH) and acetylcholine (ACH) by butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE) are studied. ATCH is used for testing of enzymatic hydrolysis of ACH in vitro, because mechanism of ATCH hydrolysisis qualitatively similar to ACH and its reaction course can be quantitatively on-line measured by two independent methods: spectrophotometrical (determination of thiocholine - product of ATCH hydrolysis - using Ellman´s method) and electrochemical (determination of acetic acid - product of ATCH hydrolysis - by pH-stat method). All tested hydrolyses correspond to the Michaelis-Menten´s equation with the second irreversible step up to the total exhaustion of the substrate. The correlations were made by means of differential and integral kinetic equations describing Michaelis-Menten model. The optimal values of Michaelis constant (KM), maximum velocity (Vm), kinetic constants of single reaction steps and absolute concentration of the used

Czech name

Kinetika hydrolýzy acetylthiocholinu a acetylcholinu pomocí cholinesteráz

Czech description

Byla sledována kinetika enzymatické hydrolýzy acetylthiocholinu a acetylcholinu cholinesterázami. Všechny testované hydrolýzy splňují až do totálního vyčerpání substrátu model Michaelis-Mentenové s druhým irreversibilním krokem. Na základě experimentálních dat byly vypočteny optimální hodnoty Michaelisovy konstanty KM, maximální rychlost Vm a kinetické konstanty jednotlivých reakčních kroků pro všechny testované systémy

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemico-Biological Interactions

ISSN

0009-2797

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

157-158

Country of publishing house

GB - UNITED KINGDOM

Number of pages

2

Pages from-to

387-388

UT code for WoS article

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EID of the result in the Scopus database

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