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Kinetics of total enzymatic hydrolysis of acetylcholine and acetylthiocholine

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004415" target="_blank" >RIV/00216275:25310/06:00004415 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Kinetics of total enzymatic hydrolysis of acetylcholine and acetylthiocholine

  • Original language description

    Kinetics and the mechanism of total in vitro hydrolyses (i.e. up to the exhaustion of substrate) of acetylcholine and acetylthiocholine by acetylcholinesterase and butyrylcholinesterase were studied in vitro in a batch reactor at 25°C, pH 8 and ionic strength of 0.11 M. Every hydrolysis was monitored by 2-3 independent analytical methods. All studied types of enzymatic hydrolyses fulfilled the Michaelis-Menten reaction scheme with the irreversible second step.

  • Czech name

    Kinetika úplné enzymatické hydrolýzy acetylcholinu a acetylthiocholinu

  • Czech description

    Kinetika a mechanismus totální enzymatické hydrolýzy (tzn. do úplného vyčerpání substrátu) acetylcholinu a acetylthiocholinu při použití acetylcholinesterázy a butyrylcholinesterázy byla studována in vitro ve vsádkovém reaktoru při 25°C, pH 8 a iontové síle 0.11 M. Každá hydrolýza byla sledována 2-3 nezávislými analytickými metodami. Všechny studované typy enzymatické hydrolýzy splňují reakční schema Michaelis-Mentenové s nevratným druhým krokem.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Zeitschrift fuer Naturforschung C-A Journal of Biosciences

  • ISSN

    0939-5075

  • e-ISSN

  • Volume of the periodical

    61c

  • Issue of the periodical within the volume

    3-4

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    6

  • Pages from-to

    289-294

  • UT code for WoS article

  • EID of the result in the Scopus database