Comparison of Various Second-Dimension Gradient Types in Comprehensive Two dimensional Liquid Chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F10%3A39882333" target="_blank" >RIV/00216275:25310/10:39882333 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of Various Second-Dimension Gradient Types in Comprehensive Two dimensional Liquid Chromatography

Original language description

Gradient elution provides significant improvement in peak capacity with respect to isocratic conditions. In the second dimension, gradients are limited to a short-time period available for separation. Various types of second-dimension gradients in comprehensive LCLC are compared: (i) ''full in fraction'', (ii) ''segment in fraction'' and (iii) ''continuously shifting'' gradients, applied in orthogonal LCLC separations of'phenolic acids and flavones on a polyethylene glycol column in the first dimensionand two types of porous shell fused-core C18 columns in the second dimension (Ascentis Express and Kinetex). The porous shell columns provide narrow bandwidths and fast second-dimension separations at moderate operating pressure that allows important savings of the overall separation time in comprehensive LCLC separations. The effects of the gradient type on the bandwidths, theoretical peak capacity, separation time and column pressure in the second dimension were investigated.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: New analytical methods in combined high-capacity phase separation systems</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

16

Pages from-to

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UT code for WoS article

000278572000005

EID of the result in the Scopus database

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