Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892169" target="_blank" >RIV/00216275:25310/11:39892169 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2010.10.095" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2010.10.095</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2010.10.095" target="_blank" >10.1016/j.chroma.2010.10.095</a>

Result language
angličtina
Original language name
Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography
Original language description
Gradient elution improve peak capacity with respect to isocratic conditions and should be preferably used in 2D LC x LC in both dimensions. Gradient conditions spanning over a broad range are used with generic "full in fraction" (FIF) gradients. "Segmentin fraction" (SIF) gradients cover a limited gradient range. Optimization of the gradient profiles is important tool for achieving high 2D peak capacity and savings of the separation time. The transferred fraction volumes may significantly affect the bandwidths, especially at high elution strength of the fraction solvent, which may cause even band distortion or splitting in combined normal-phase (HILIC)-RP systems, but also in some 2D RP-RP systems. In the present work, the effects of the fast gradientprofile, of the sample volume and solvent on the elution time and bandwidths were investigated on a short column packed with fused-core porous-shell particles, providing narrow bandwidths and fast separations at moderate operating pressu
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: New analytical methods in combined high-capacity phase separation systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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