Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896383" target="_blank" >RIV/00216275:25310/13:39896383 - isvavai.cz</a>
Result on the web
<a href="http://www.electrochemsci.org/papers/vol8/80709030.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol8/80709030.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity
Original language description
An amperometric biosensor for the determination of hydroquinone and other polyphenolic antioxidants is presented, based on a carbon paste electrode on which tyrosinase is immobilized in a Nafion (R) film. In a 0.1 M phosphate buffer (pH 7.0 and 25 +/- 1degrees C), the antioxidant capacity can be expressed as the concentration equivalent of p-hydroquinone (HQ), according to an amperometric response of polyphenolic antioxidants with the carbon paste sensor at -0.24 V vs. Ag/AgCl/3 M KCl. In the range oflow HQ concentrations (up to 120 mu mol L-1), the corresponding calibration curve follows the equation I [mu A] = -0.0426 -0.0113 c(HQ) [mu mol L-1] with a correlation coefficient R = 0.999. The limit of detection (LOD for S/N = 3) was estimated as 1.6 mu mol L-1, the repeatability was 1.2 % RSD (c = 90 mu M, n = 5 measurements). The biosensor can be applied to determine the phenolic antioxidant capacity in plants and food samples in the absence of higher concentrations of ascorbic acid.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Electrochemical Science
ISSN
1452-3981
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
7
Country of publishing house
CS - SERBIA AND MONTENEGRO
Number of pages
11
Pages from-to
9030-9040
UT code for WoS article
000323547600010
EID of the result in the Scopus database
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