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Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898288" target="_blank" >RIV/00216275:25310/14:39898288 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Retention characteristics of labelled oligosaccharides in hydrophilic interaction liquid chromatography

  • Original language description

    Conditions of hydrophilic interaction liquid chromatopraphy of labelled oligosaccharides (columns, gradient profiles, mobile phase flow and additives) were optimized with the aim of coupling it to the capillary zone electrophoresis in two-dimensional system. Different common derivatization reagents used for fluorescent labeling of oligosaccharides including 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) we compared. For a separation of labelled oligosaccharides, microcolumns with different stationary phases were tested (Silica gel, Amine, TSK gel amide-80 and GlycanPac stationary phases) Mass spectrometer was used for detection.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA14-06319S" target="_blank" >GA14-06319S: Development of microfluidic interfaces for multidimensional liquid phase separation/mass spectrometry analysis of complex biological samples</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis

  • ISBN

    978-80-904959-2-0

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    440-443

  • Publisher name

    Ústav analytické chemie AV ČR, v. v. i.

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Oct 20, 2014

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article