Assay of total glutathione and glutathione disulphide in seminal plasma of male partners of couples presenting for a fertility evaluation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898311" target="_blank" >RIV/00216275:25310/14:39898311 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/and.12176" target="_blank" >http://dx.doi.org/10.1111/and.12176</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/and.12176" target="_blank" >10.1111/and.12176</a>
Alternative languages
Result language
angličtina
Original language name
Assay of total glutathione and glutathione disulphide in seminal plasma of male partners of couples presenting for a fertility evaluation
Original language description
A method is described here for the determination of total glutathione (TGSH) and glutathione disulphide (GSSG) in the seminal plasma of the male partners of couples requesting a fertility evaluation. A suitable sample preparation procedure prior to high-performance liquid chromatography analysis is discussed. After adequate sample preparation, the samples were derivatised with ortho-phthaldialdehyde to form a stable, highly fluorescent tricyclic derivative. Reversed-phase column chromatography was usedfor the separation, and the effluent was monitored with a fluorescence detector at an excitation wavelength of 350 nm and an emission wavelength of 420 nm. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 94.1% (CV 2.3%) for TGSH and 93.2% (CV 4.0%) for GSSG. No significant differences were found in either TGSH or GSSG concentration between the smokers and nonsmokers (2.07
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Andrologia
ISSN
0303-4569
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1079-1088
UT code for WoS article
000344913900001
EID of the result in the Scopus database
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