An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896347" target="_blank" >RIV/00216275:25310/13:39896347 - isvavai.cz</a>

Result on the web

<a href="http://www.tandfonline.com/doi/full/10.1080/10826076.2012.706858#.Urf2j7TtAg4" target="_blank" >http://www.tandfonline.com/doi/full/10.1080/10826076.2012.706858#.Urf2j7TtAg4</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

An assay of total glutathione and glutathione disulfide in human whole blood and plasma using a high-performance liquid chromatography with fluorescence detection

Original language description

A method is described here for the determination of total glutathione and glutathione disulfide in human whole blood and plasma with a suitable sample preparation procedure to prevent glutathione oxidation and glutathione disulfide hydrolysis. Whole blood samples were obtained from a group of blood donors. After adequate sample preparation, the samples were derivatized with ortho-phthaldialdehyde to form a stable, highly fluorescent tricyclic derivative. Reverse-phase column chromatography was used forthe separation and fluorimetric detection to monitor the effluent. The analytical performance of this method was satisfactory for the determination of total glutathione and glutathione disulfide. The intra-assay and inter-assay coefficients of variationwere below 10%. The recoveries were as follows: 96.1% (CV 1.4%) and 104.3% (CV 2.4%) for whole blood, 92.3% (CV 2.4%) and 107.4% (CV 2.6%) for plasma. We found no significant differences in both total glutathione and glutathione disulfide

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Liquid Chromatography & Related Technologies

ISSN

1082-6076

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

14

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

2013-2028

UT code for WoS article

000319553100009

EID of the result in the Scopus database

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