Comparison of Mass Spectrometry Based Approaches in Lipidomics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899340" target="_blank" >RIV/00216275:25310/14:39899340 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of Mass Spectrometry Based Approaches in Lipidomics
Original language description
Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitativ
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů