Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900260" target="_blank" >RIV/00216275:25310/15:39900260 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/15:00448631

Result on the web

<a href="http://dx.doi.org/10.1039/c5lc00224a" target="_blank" >http://dx.doi.org/10.1039/c5lc00224a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c5lc00224a" target="_blank" >10.1039/c5lc00224a</a>

Result language

angličtina

Original language name

Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors

Original language description

A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol-ene chemistry is presented. The method allows monolith synthesis and anchoring inside thiol-ene microchannels in a single photoinitiated step. Characterization by scanning electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol-ene beads anchored solidly inside thiol-ene microchannels. Surfacearea measurements indicate that the monoliths are macroporous, with no or little micro- or mesopores. As a demonstration, galactose oxidase and peptide-N-glycosidase F (PNGase F) were immobilized at the surface of the synthesized thiol-ene monoliths viatwo different mechanisms. First, cysteine groups on the protein surface were used for reversible covalent linkage to free thiol functional groups on the monoliths. Second, covalent linkage was achieved via free primary amino groups on the

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Lab On a Chip

ISSN

1473-0197

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

2162-2172

UT code for WoS article

000354196600002

EID of the result in the Scopus database

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