Extraction, separation and identification of bioactive compounds in barley using LC-MS/MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901783" target="_blank" >RIV/00216275:25310/16:39901783 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Extraction, separation and identification of bioactive compounds in barley using LC-MS/MS
Original language description
An extraction procedure, based on alkaline and acidic hydrolysis, was developed, using the Box-Behnken design of experiment, with the aim to achieve an exhaustive recovery of free and also linked polyphenols, i.e. bonded to cell structures, in barley (Hordeum Vulgare L.) samples. An LC-MS/MS (MRM transition mode) method was developed for analysis of the samples extracted through the optimized procedure, and to achieve a correct quali-quantitative characterization of a specific group of polyphenols. The optimized procedure was also employed for the extraction and the identification of bioactive molecules in a dietary supplement composed of dried leaves of barley, commonly known as "young barley". A selectivity study was carried out employing some of the compounds identified in young barley, and by using six different columns (C18, silica, ES-CN and OH5 types). The results showed that different mechanisms are involved in the retention of the standards, especially in case of HILIC mode.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
CECE 2016 : 13th International Interdisciplinary Meeting on Bioanalysis
ISBN
978-80-904959-4-4
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
59-62
Publisher name
Ústav analytické chemie AV ČR, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Oct 17, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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