Voltammetric determination of cholecalciferol at glassy carbon electrode performed in water-ethanol mixture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39912547" target="_blank" >RIV/00216275:25310/18:39912547 - isvavai.cz</a>

Result on the web

<a href="http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/viewFile/889/pdf/" target="_blank" >http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/viewFile/889/pdf/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5219/889" target="_blank" >10.5219/889</a>

Result language

angličtina

Original language name

Voltammetric determination of cholecalciferol at glassy carbon electrode performed in water-ethanol mixture

Original language description

To confirm or disprove previous hypotheses, cyclic voltammetry of 0.5 mM cholecalciferol (vitamin D3) at glassy carbon electrode (GCE) and platinum disk electrode (PtE) in pure acetonitrile and water-ethanol mixture at 50 mV·s-1 has been used to investigate the oxidation mechanism. The oxidation occurs in two one-electrone steps. According to calculation of the highest electron density in cholecalciferol molecule which is evidently delocalized over carbon atoms of the three conjugated double bonds (C19, C10, C5-C8) points to part of the molecule involved in oxidation processes. An oxidation peak (at +0.925 V vs. Ag/AgCl) was used to develop direct voltammetric method based on differential pulse voltammetry for the vitamin D3 determination at GCE performed in 40% ethanol containing 0.1 M LiClO4. Under optimization of analytical procedure, it was found that a composition of the supporting electrolyte used significantly affects a current response of oxidation peak obtained. Satisfactory sensitivity was achieved in the 1:1 water-ethanol mixture containing 0.05 M lithium perchlorate as as supporting electrolyte. The linear range for vitamin D3 determination was 2.4 × 10−6 – 3.5 × 10−4 M with the detection limit of 8.0 × 10−7 M. This work demonstrates a fact that the GCE is suitable electroanalytical device for analysis of various food supplements and medicaments.

Czech name

—

Czech description

—

Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Potravinarstvo

ISSN

1338-0230

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

1

Country of publishing house

SK - SLOVAKIA

Number of pages

7

Pages from-to

166-172

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85043483452