Reversed phase UHPLC/ESI-MS determination of oxylipins in human plasma: a case study of female breast cancer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F19%3A39914784" target="_blank" >RIV/00216275:25310/19:39914784 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/19:73596368 RIV/70883521:28150/19:63523380
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs00216-018-1556-y" target="_blank" >https://link.springer.com/article/10.1007%2Fs00216-018-1556-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-018-1556-y" target="_blank" >10.1007/s00216-018-1556-y</a>
Alternative languages
Result language
angličtina
Original language name
Reversed phase UHPLC/ESI-MS determination of oxylipins in human plasma: a case study of female breast cancer
Original language description
The ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC/MS) method was optimized and validated for the determination of oxylipins in human plasma using the targeted approach with selected reaction monitoring (SRM) in the negative-ion electrospray ionization (ESI) mode. Reversed phase UHPLC separation on an octadecylsilica column enabled the analysis of 63 oxylipins including numerous isomeric species within 12-min run time. The method was validated (calibration curve, linearity, limit of detection, limit of quantification, carry-over, precision, accuracy, recovery rate, and matrix effect) and applied to 40 human female plasma samples from breast cancer patients and age-matched healthy volunteers (control). Thirty-six oxylipins were detected in human plasma with concentrations above the limit of detection, and 21 of them were quantified with concentrations above the limit of quantitation. The concentrations determined in healthy controls are in a good agreement with previously reported data on human plasma. Quantitative data were statistically evaluated by multivariate data analysis (MDA) methods including principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA). S-plot and box plots showed that 13-HODE, 9-HODE, 13-HOTrE, 9-HOTrE, and 12-HHTrE were the most upregulated oxylipin species in plasma of breast cancer patients.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
411
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
13
Pages from-to
1239-1251
UT code for WoS article
000458358400012
EID of the result in the Scopus database
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