Determination of selected alpha-keto acids in dried blood samples using HPLC with fluorescence detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39919066" target="_blank" >RIV/00216275:25310/22:39919066 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0731708522001595?token=3F716DF414A2A118CA22E35E713AE0D10EA5D0153B97DF6DA99A53B1C615E6B42DFAC79E26163B81A078B729588F7F49&originRegion=eu-west-1&originCreation=20221208125816" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0731708522001595?token=3F716DF414A2A118CA22E35E713AE0D10EA5D0153B97DF6DA99A53B1C615E6B42DFAC79E26163B81A078B729588F7F49&originRegion=eu-west-1&originCreation=20221208125816</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2022.114738" target="_blank" >10.1016/j.jpba.2022.114738</a>

Result language
angličtina
Original language name
Determination of selected alpha-keto acids in dried blood samples using HPLC with fluorescence detection
Original language description
The determination of alpha-keto acids derived from amino acids is currently the most reliable approach for the diagnosis of some congenital metabolic diseases. An HPLC method for the simultaneous measurement of selected alpha-keto acids in dried blood samples has been developed and evaluated. Blood spot samples from a group of healthy blood donors were collected onto #903 Specimen Collection Paper. Prior the separation, the alpha-keto acids were derivatized with 1,2-diamino-4,5-dimethoxybenzene to the corresponding 3-substituted-6,7-dimethoxy-2 (1 H)-quinoxalinol derivatives. For the separation, a reverse-phase column LichroCart 125-4, Purospher RP-18e, 5 mu m, was used. The mixture of 25% ACN in deionized water (mobile phase A) and 100% ACN (mobile phase B) were used for a gradient elution of alpha-keto acids derivatives. Analytical performance of this method is satisfactory for all alpha-keto acids. The intra-assay and inter-assay coefficients were below 10% and recoveries were close to 100%. We have developed relatively simple, rapid, selective and sufficiently sensitive HPLC method with fluorescence detection for the determination of selected alpha-keto acids in dried blood samples. The presented method is suitable for clinical testing purposes.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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