Application of magnetic carriers for whole DNA isolation and detection of probiotic Lactobacillus gasseri K7 strain in complex samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU83416" target="_blank" >RIV/00216305:26310/09:PU83416 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Application of magnetic carriers for whole DNA isolation and detection of probiotic Lactobacillus gasseri K7 strain in complex samples

Original language description

The importance of lactic acid bacteria (LAB) for dairy industry is well known. Increasing interest has been focused on some LAB strains that are proposed to be probiotic. Lactobacillus gasseri K7 is a probiotic strain, producer of gassericins K7 A and B1, which was tested in vivo in mice. Falsely negative results can occur in PCR identification of target cells due to the presence of extracellular PCR inhibitors in tested samples - mouse faeces. The problem can be solved by solid phase systems adsorbinggenomic DNA. Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl dimethylmethacrylate) (P(HEMA-co-GMA) were used for the isolation of whole DNA from mouse faeces. These microspheres reversibly bind DNA in the presence of high concentrations of poly(ethylene glycol) (PEG 6000) and sodium chloride2. The release of adsorbed DNA from the microsphereses was optimalised using a low-ionic-strength TE buffer of different pH and temperature. The presence of t

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Gastro jenterologia

ISSN

1727-7906

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

4

Country of publishing house

RU - RUSSIAN FEDERATION

Number of pages

1

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—