Magnetic hydrophilic microspheres P (HEMA-co-GMA) for DNA isolation from mouse faeces
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU83437" target="_blank" >RIV/00216305:26310/09:PU83437 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Magnetic hydrophilic microspheres P (HEMA-co-GMA) for DNA isolation from mouse faeces
Original language description
Probiotic lactic acid bacteria (LAC) play an important role in health maintenance of the human gastrointestinal tract. A mouse model is widely used for the study of LAC influence on the organism's health. Polymerase chain reaction (PCR) has become a powerful diagnostic tool for the analysis of microorganisms in different types of samples. Faecal samples are a difficult specimen for the application of PCR due to the presence of PCR inhibitors. For this reason an optimal DNA extraction method is crucial for the identification of microorganisms in faecal samples. The problem can be solved by reversible adsorption of whole DNA on magnetic particle surface in the presence of PEG 6000 and sodium chloride. Magnetic non-porous hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres containing carboxyl groups were used for this purpose (Rittich et al. 2009). In this work the proposed method was used for DNA isolation from mouse faeces and identificat
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of Abstract 4th International Symposium on RECENT ADVANCES IN FOOD ANALYSIS
ISBN
978-80-7080-726-2
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
Neuveden
Place of publication
Praha
Event location
Praha - hotel Diplomat
Event date
Nov 4, 2009
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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