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Charge transfer in DNA and RNA: dependence on the temperature

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F12%3APU102933" target="_blank" >RIV/00216305:26310/12:PU102933 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Charge transfer in DNA and RNA: dependence on the temperature

  • Original language description

    DNA and RNA are compounds, very familiar to us. They are key to understanding of living matrix. All important processes, including the most fascinating such as the coding the amino-acids, are accompanied by charge transfers between each nucleotide. The range of the charge can be recorded on the basis of various measurements, for example the fluorescence, which depends on many conditions. In this case, we used steady state fluorescence to determine the emission and excitation spectras and Time Correlated Single Photon Counting method to determine lifetime of the fluorescence. Four samples of synthetized oligomers of twelve nucleotides with 2-aminopurine as electron acceptor were analyzed. To characterize the influence of temperature on charge transfer, redox-active samples of DNA-DNA and RNA-DNA were compared with reference redox-inactive counterparts. The redox-active oligomers have guanine as electron donor separated by two A-T or A-U basepairs. From the excitation spectras and differences in the 270-280nm region we describe the influence of temperature on the extent of base stacking of 2-aminopurine to the chain. From the decrease of the redox active oligomers fluorescence relative to the redox-inactive counterparts, the extent of charge transfer is determined and related to the temperature. The results of these measurements might positively contribute to the further research in the field of biosensoric medicine and for better and more precise understanding of human body processes.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Chemie je život

  • ISBN

    978-80-214-4644-1

  • ISSN

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    82-87

  • Publisher name

    Vysoké učení technické v Brně, Fakulta chemická

  • Place of publication

    Purkyňova 464/118, 612 00 Brno

  • Event location

    Brno

  • Event date

    Dec 7, 2012

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article