Charge transfer in DNA and RNA: dependence on the temperature
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F12%3APU102933" target="_blank" >RIV/00216305:26310/12:PU102933 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Charge transfer in DNA and RNA: dependence on the temperature
Original language description
DNA and RNA are compounds, very familiar to us. They are key to understanding of living matrix. All important processes, including the most fascinating such as the coding the amino-acids, are accompanied by charge transfers between each nucleotide. The range of the charge can be recorded on the basis of various measurements, for example the fluorescence, which depends on many conditions. In this case, we used steady state fluorescence to determine the emission and excitation spectras and Time Correlated Single Photon Counting method to determine lifetime of the fluorescence. Four samples of synthetized oligomers of twelve nucleotides with 2-aminopurine as electron acceptor were analyzed. To characterize the influence of temperature on charge transfer, redox-active samples of DNA-DNA and RNA-DNA were compared with reference redox-inactive counterparts. The redox-active oligomers have guanine as electron donor separated by two A-T or A-U basepairs. From the excitation spectras and differences in the 270-280nm region we describe the influence of temperature on the extent of base stacking of 2-aminopurine to the chain. From the decrease of the redox active oligomers fluorescence relative to the redox-inactive counterparts, the extent of charge transfer is determined and related to the temperature. The results of these measurements might positively contribute to the further research in the field of biosensoric medicine and for better and more precise understanding of human body processes.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Chemie je život
ISBN
978-80-214-4644-1
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
82-87
Publisher name
Vysoké učení technické v Brně, Fakulta chemická
Place of publication
Purkyňova 464/118, 612 00 Brno
Event location
Brno
Event date
Dec 7, 2012
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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