High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU106482" target="_blank" >RIV/00216305:26310/13:PU106482 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations
Original language description
In this work we have modified our concept of simple splitless gradient liquid chromatographic system for micro and nano columns with backpressure up to 50 bar. Presented configuration allows us to run analysis under the pressure up to 400 bar. Experimental configuration consists of an OEM programmable syringe pump equipped with a 250-ul high-pressure syringe and ten-port selector valve. High pressure syringe is connected to central port of selector valve via 0.5 meter long PEEK capillary of 380 um i. d.fixed in horizontal plane. PEEK capillary here serves as gradient generator as well as gradient loop and as sample loop. Mobile phase gradients are created by successive sucking of defined volumes of four water-acetonitrile solutions with gradually changing acetonitrile concentration into the PEEK capillary. Sharp mobile phase boundaries fade out while passing through the valve body and due to the parabolic profile of laminar flow as well as due to diffusion. Available linear gradients
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemické listy
ISSN
1213-7103
e-ISSN
—
Volume of the periodical
107
Issue of the periodical within the volume
s3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
3
Pages from-to
438-440
UT code for WoS article
—
EID of the result in the Scopus database
—