Semi-quantitative estimation of soya protein-based additives in meat products using real-time polymerase chain reaction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU136123" target="_blank" >RIV/00216305:26310/15:PU136123 - isvavai.cz</a>
Result on the web
<a href="http://agris.fao.org/agris-search/search.do?recordID=SK2016013970" target="_blank" >http://agris.fao.org/agris-search/search.do?recordID=SK2016013970</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Semi-quantitative estimation of soya protein-based additives in meat products using real-time polymerase chain reaction
Original language description
A real-time polymerase chain reaction (PCR) detection system with a TaqMan probe for quantification of soya in meat products was developed and evaluated. The detection system targeted the Le1 gene encoding for soya lectin and exhibited 100% inclusivity (9 Glycine max cultivars) and 100% exclusivity (22 plant samples) for the detection of soya. Analytical sensitivity of the assay was 5.75 pg DNA per reaction, which corresponds to approx. 2 haploid genome equivalents. Practical detection and quantification limits for three different soya protein-based additives (protein concentrate, protein isolate, okara), in liver pate as a matrix, were at a level of 0.1 g.kg(-1). The calibration lines for each series of model samples containing individual soya protein-based additives were statistically identical in the range from limit of quantification to 100 g.kg(-1), which means that the same calibration line could be used for all soya protein-based additives. The developed real-time PCR facilitated semi-quantitative estimation of soya protein-based additives in meat products, which may be useful for the control of labelling of meat products.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21101 - Food and beverages

Project
<a href="/en/project/LO1211" target="_blank" >LO1211: Materials Research Centre at FCH BUT- Sustainability and Development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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