Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU133547" target="_blank" >RIV/00216305:26310/19:PU133547 - isvavai.cz</a>
Result on the web
<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=12859950138" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=12859950138</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.
Original language description
In this contribution, the process of sampling the DBSs and their subsequent elution with deionized water, methanol, acetonitrile, and optionally with mixtures thereof is shown. Various effects of the co-eluted matrix components on CE-C4D were observed for the selected elution solutions and are comprehensively described. The CE-C4D method was optimized for baseline separation of biogenic amino acids and indicated excellent analytical parameters and high stability of the separation system. CE-C4D was used for the direct analysis of the DBS eluates from healthy donors with amino acid concentrations at physiological levels. Finally, the method was applied to the determination of selected amino acids from DBS samples, which are related to common inborn metabolic disorders, such as maple syrup urine disease and phenylketonuria.
Czech name
—
Czech description
—

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)