Nanostructured Dual-Delivery System with Antioxidant and Synergistic Approach for Targeted Dermal Treatment
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F26%3A0199697" target="_blank" >RIV/00216305:26310/26:0199697 - isvavai.cz</a>
Result on the web
<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:001594838800001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:001594838800001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/IJMS26199485" target="_blank" >10.3390/IJMS26199485</a>
Alternative languages
Result language
angličtina
Original language name
Nanostructured Dual-Delivery System with Antioxidant and Synergistic Approach for Targeted Dermal Treatment
Original language description
Biocompatible nanofibrous dressings integrating bioactive compounds with antioxidative and antimicrobial properties offer a promising solution for effective wound healing. In the presented study, we developed a novel dual-delivery system by combining forcespun nanofibres with poly(3-hydroxybutyrate) (PHB)-liposomes to enhance bioavailability and enable targeted release of bioactive agents (eugenol, thymol, curcumin, ampicillin, streptomycin, gentamicin). These agents exhibited notable antioxidant activity (2.27-2.33 mmol TE/g) and synergistic or partially synergistic antimicrobial effects against E. coli, M. luteus, S. epidermidis, and P. aeruginosa ( Fractional Inhibitory Concentration index 0.09-0.73). The most potent combinations, particularly thymol, eugenol, and ampicillin, were encapsulated in the nanofibre-liposomal matrix. The successful preparation of a new combined delivery system was confirmed by structural analysis using Electron and Fluorescence Microscopy. The dual-composite materials retained the antimicrobial properties of the individual compounds upon release, with the highest increases of similar to 73.56% against S. epidermidis. Cell viability and in vitro immunology assays using the human keratinocyte cell line (HaCaT) showed a slight decrease in viability and immune response stimulation, while not impairing wound re-epithelisation. These findings highlight the potential of firstly reported novel carrier utilising both PHB-nanofibres and PHB-liposomes, exhibiting simultaneous antioxidant and antimicrobial activity as promising candidates for the treatment of infected wounds under oxidative stress.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1661-6596
e-ISSN
1422-0067
Volume of the periodical
26
Issue of the periodical within the volume
19
Country of publishing house
CH - SWITZERLAND
Number of pages
28
Pages from-to
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UT code for WoS article
001594838800001
EID of the result in the Scopus database
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