STUDY OF COMPLEXES OF ANIMAL METAL-BINDING PROTEIN WITH PLATINUM CYTOSTATICS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F12%3APU126961" target="_blank" >RIV/00216305:26620/12:PU126961 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

STUDY OF COMPLEXES OF ANIMAL METAL-BINDING PROTEIN WITH PLATINUM CYTOSTATICS

Original language description

The suggestion of interactions between heavy metals and biologic active molecules haven't been exactly estimated yet. However cadmium, lead or mercury are significant environment pollutants and platinum and arsenic are used as oncologic medicament, they have common characteristic. In organism these compounds are not volatile but they are bounded to other molecules. Interactions between heavy metals and proteins are important for range of physiologic processes like transpiration, photosynthesis or detoxification of organisms. In our experiment an electrochemical profile of interactions between 23 metallothionein fragments and cisplatin was studied. At first 23 MT fragments (decapeptides) were selected given by differences in aminoacids ordering. For the experiment amperometric detection implemented to flow injection analysis system (FIA-ED) was used. However a lot of results were estimated, we focused on complex formation between cisplatin and 23 MT fragments analyzed in various conditions. All the 23 MT fragments interacted with cisplatin, in the optional conditions as equimolar ratio, in physiological conditions of phosphate buffer (pH 7.5) in temperature of 37 degrees C. Based on results obtained we determined an interaction constant which defines an ability of each peptide to make an interaction with cisplatin. The highest IC was found by fragments 18 and 22 and the lowest IC by 1, 15, 12 and 19. We found that the major influence of interaction was done not by the change of near neighbouring aminoacids with the conservative cysteines byt these which were about 2 or 3 of positions far away from cysteiene.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MENDELNET 2012

ISBN

978-80-7375-836-3

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

1265-1271

Publisher name

Neuveden

Place of publication

Neuveden

Event location

Mendel Univ, Fac Agron, Brno

Event date

Nov 21, 2012

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

000366461200148