Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU125847" target="_blank" >RIV/00216305:26620/16:PU125847 - isvavai.cz</a>
Result on the web
<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2209322" target="_blank" >10.1117/12.2209322</a>
Alternative languages
Result language
angličtina
Original language name
Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media
Original language description
In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
QUANTITATIVE PHASE IMAGING II
ISBN
978-1-62841-952-8
ISSN
0277-786X
e-ISSN
—
Number of pages
5
Pages from-to
„97181Y-1“-„97181Y-5“
Publisher name
Neuveden
Place of publication
Neuveden
Event location
San Francisco
Event date
Feb 13, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000380605300044