Electrochemical and spectral behaviour of miR-34a-5p
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU126041" target="_blank" >RIV/00216305:26620/16:PU126041 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00706-015-1600-y" target="_blank" >http://dx.doi.org/10.1007/s00706-015-1600-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-015-1600-y" target="_blank" >10.1007/s00706-015-1600-y</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical and spectral behaviour of miR-34a-5p
Original language description
MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA -> miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3-6.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XXXV. Moderní elektrochemické metody : sborník přednášek mezinárodní odborné konference
ISBN
978-80-905221-3-8
ISSN
0026-9247
e-ISSN
1434-4475
Number of pages
6
Pages from-to
105-110
Publisher name
Neuveden
Place of publication
Neuveden
Event location
Jetřichovice
Event date
May 18, 2015
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
000367521400014