Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134919" target="_blank" >RIV/00216305:26620/19:PU134919 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917134
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines
Original language description
This study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/GC19-13766J" target="_blank" >GC19-13766J: Zinc signaling and metallothioneins sub/isoforms expression in breast cancer: implications for prognostic and therapeutic purposes</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
606-611
Publisher name
Mendel University in Brno
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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