All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134919" target="_blank" >RIV/00216305:26620/19:PU134919 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/19:43917134

  • Result on the web

    <a href="https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

  • Original language description

    This study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    30204 - Oncology

Result continuities

  • Project

    <a href="/en/project/GC19-13766J" target="_blank" >GC19-13766J: Zinc signaling and metallothioneins sub/isoforms expression in breast cancer: implications for prognostic and therapeutic purposes</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2019 Proceedings of 26th International PhD Students Conference

  • ISBN

    978-80-7509-688-3

  • ISSN

  • e-ISSN

  • Number of pages

    6

  • Pages from-to

    606-611

  • Publisher name

    Mendel University in Brno

  • Place of publication

    Neuveden

  • Event location

    Brno

  • Event date

    Nov 6, 2019

  • Type of event by nationality

    CST - Celostátní akce

  • UT code for WoS article