Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F23%3APU146944" target="_blank" >RIV/00216305:26620/23:PU146944 - isvavai.cz</a>

Result on the web

<a href="https://www.webofscience.com/wos/woscc/full-record/WOS:000924683700001" target="_blank" >https://www.webofscience.com/wos/woscc/full-record/WOS:000924683700001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apmt.2023.101736" target="_blank" >10.1016/j.apmt.2023.101736</a>

Result language

angličtina

Original language name

Demolded hollow high aspect-ratio parylene-C micropillars for real-time mechanosensing applications

Original language description

Cells generate mechanical forces to maintain normal cellular function or play a role in developing pathological processes. The mechanical force called the traction force can be estimated from the pillar deflection using a polymeric micropillar array. Here, we develop a transparent membrane of hollow coneshape high aspect ratio (AR) parylene-C micropillars by the molding method. The membrane of the pillar array is exposed after the etching of the silicon mold and the residual silicon serves as the cultivation chamber. The AR and spring constant (k) of micropillars are estimated to AR ≈ 10 and k ≈ 0.349 N·m−1. The spring constant of developing micropillars is 3.5-times decreased compared to cylindrical, non-hollow pillars. This slightly tune the elastic properties of micropillars. The array is further shown as the mechanosensor detecting the change of cellular tension during hyperosmotic stress. The traction force of cancerous PC-3 cells is estimated from pillar deflections by image analysis. Additionally, the cell volume and surface area are measured using a digital holographic microscope (DHM). The results show that the molding technique can be used to develop high AR parylene-C micropillars and that this array can serve as the mechanosensor of cellular processes.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Materials Today

ISSN

2352-9407

e-ISSN

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Volume of the periodical

31

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

1-12

UT code for WoS article

000924683700001

EID of the result in the Scopus database

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