On quantitativeness of diffraction-limited quantitative phase imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU155954" target="_blank" >RIV/00216305:26620/24:PU155954 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/24:73627004

Result on the web

<a href="https://pubs.aip.org/aip/app/article/9/12/126111/3325120/On-quantitativeness-of-diffraction-limited" target="_blank" >https://pubs.aip.org/aip/app/article/9/12/126111/3325120/On-quantitativeness-of-diffraction-limited</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/5.0232405" target="_blank" >10.1063/5.0232405</a>

Result language

angličtina

Original language name

On quantitativeness of diffraction-limited quantitative phase imaging

Original language description

Quantitative phase imaging (QPI) has advanced by accurately quantifying phase shifts caused by weakly absorbing biological and artificial structures. Despite extensive research, the diffraction limits of QPI have not been established and examined. Hence, it remains unclear whether diffraction-affected QPI provides reliable quantification or merely visualizes phase objects, similar to phase contrast methods. Here, we develop a general diffraction phase imaging theory and show that it is intrinsically connected with Rayleigh's resolution theory. Our approach reveals the entanglement of phases under restoration, imposing diffraction bounds on spatial phase resolution and, unexpectedly, on phase accuracy. We prove that the phase accuracy depends on the size, shape, and absorption of objects forming the sample and significantly declines if the object size approaches the Rayleigh limit (a relative phase error of -16% for an Airy disk-sized object with low phase shift). We show that the phase accuracy limits can be enhanced at the cost of deteriorated phase resolution by attenuating the sample background light. The QPI diffraction limits are thoroughly examined in experiments with certified phase targets and biological cells. The study's relevance is underscored by results showing that the phase accuracy of some structures is lost (a relative phase error of -40%) even though they are spatially resolved (a phase visibility of 0.5). A reliable procedure is used to estimate phase errors in given experimental conditions, opening the way to mitigate errors' impact through data post-processing. Finally, the phase accuracy enhancement in super-resolution QPI is discovered, which has not been previously reported.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10306 - Optics (including laser optics and quantum optics)

Project

<a href="/en/project/LM2023050" target="_blank" >LM2023050: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

APL Photonics

ISSN

2378-0967

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

„126111-1“-„126111-14“

UT code for WoS article

001379528800001

EID of the result in the Scopus database

2-s2.0-85211984837