Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F22%3A10444226" target="_blank" >RIV/00669806:_____/22:10444226 - isvavai.cz</a>
Alternative codes found
RIV/49777513:23520/22:43965086 RIV/00216208:11140/22:10444226
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bSyslppJPV" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bSyslppJPV</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fbioe.2022.833244" target="_blank" >10.3389/fbioe.2022.833244</a>
Alternative languages
Result language
angličtina
Original language name
Decellularization of Porcine Carotid Arteries: From the Vessel to the High-Quality Scaffold in Five Hours
Original language description
The use of biologically derived vessels as small-diameter vascular grafts in vascular diseases is currently intensely studied. Vessel decellularization provides a biocompatible scaffold with very low immunogenicity that avoids immunosuppression after transplantation. Good scaffold preservation is important as it facilitates successful cell repopulation. In addition, mechanical characteristics have to be carefully evaluated when the graft is intended to be used as an artery due to the high pressures the vessel is subjected to. Here, we present a new and fast decellularization protocol for porcine carotid arteries, followed by investigation of the quality of obtained vessel scaffolds in terms of maintenance of important extracellular matrix components, mechanical resistance, and compatibility with human endothelial cells. Our results evidence that our decellularization protocol minimally alters both the presence of scaffold proteins and their mechanical behavior and human endothelial cells could adhere to the scaffold in vitro. We conclude that if a suitable protocol is used, a high-quality decellularized arterial scaffold of non-human origin can be promptly obtained, having a great potential to be recellularized and used as an arterial graft in transplantation medicine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Bioengineering and Biotechnology
ISSN
2296-4185
e-ISSN
2296-4185
Volume of the periodical
10
Issue of the periodical within the volume
May
Country of publishing house
CH - SWITZERLAND
Number of pages
18
Pages from-to
833244
UT code for WoS article
000803605100001
EID of the result in the Scopus database
2-s2.0-85131443955