Faecal bacteriome and metabolome profiles associated with decreased mucosal inflammatory activity upon anti-TNF therapy in paediatric Crohn's disease
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F24%3A10466302" target="_blank" >RIV/00669806:_____/24:10466302 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/24:98635 RIV/00216208:11110/24:10466302 RIV/00216208:11130/24:10466302 RIV/00216208:11140/24:10466302 and 4 more
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Fuq4Ssc90p" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Fuq4Ssc90p</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/ecco-jcc/jjad126" target="_blank" >10.1093/ecco-jcc/jjad126</a>
Alternative languages
Result language
angličtina
Original language name
Faecal bacteriome and metabolome profiles associated with decreased mucosal inflammatory activity upon anti-TNF therapy in paediatric Crohn's disease
Original language description
BACKGROUND AND AIMS: Treatment by anti-TNFα antibodies (anti-TNF) changes the dysbiotic faecal bacteriome in Crohn's disease (CD). However, it is not known whether these changes are due to decreasing mucosal inflammatory activity or whether similar bacteriome reactions might be observed in gut-healthy subjects. Therefore, we explored changes in faecal bacteriome and metabolome upon anti-TNF administration (and therapeutic response) in children with CD and contrasted those to anti-TNF-treated children with juvenile idiopathic arthritis (JIA). METHODS: Faecal samples collected longitudinally before and during anti-TNF therapy were analysed for bacteriome by massively parallel sequencing of the 16S rDNA (V4 region) and for faecal metabolome by 1H nuclear magnetic resonance. The response to treatment by mucosal healing was assessed by MINI index at three months after the treatment started. We also tested several representative gut bacterial strains for in-vitro growth inhibition by infliximab. RESULTS: We analysed 530 stool samples from 121 children (CD 54, JIA 18, healthy 49). Bacterial community composition reacted on anti-TNF in CD: three members of class Clostridia increased on anti-TNF, whereas class Bacteroidia decreased. Among faecal metabolites, glucose and glycerol increased, whereas isoleucine and uracil decreased. Some of these changes differed by treatment response (mucosal healing) after anti-TNF. No significant changes in bacteriome or metabolome were noted upon anti-TNF in JIA. Bacterial growth was not affected by infliximab in a disc diffusion test. CONCLUSIONS: Our findings suggest that gut mucosal healing is responsible for the bacteriome and metabolome changes observed in CD, rather than any general effect of anti-TNF.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30209 - Paediatrics
Result continuities
Project
<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Crohn's and Colitis
ISSN
1873-9946
e-ISSN
1876-4479
Volume of the periodical
18
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
15
Pages from-to
106-120
UT code for WoS article
001078501800001
EID of the result in the Scopus database
2-s2.0-85183581822