Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F11%3A00102039" target="_blank" >RIV/00843989:_____/11:00102039 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement

Original language description

Background: We compared two different agarose isoelectric focusing methods for detection of oligoclonal IgG bands in cerebrospinal fluid and serum: commercial method with immunofixation (Sebia) and home-made method using Multiphor II apparatus followed by affinity immunoblotting. Interobserver agreement for both methods was tested concerning the presence of intrathecal IgG synthesis, the detailed isoelectric focusing pattern type, the number of CSF-restricted oligoclonal IgG bands, and the number of oligoclonal IgG bands in CSF and in serum. Findings: Using kappa statistics for evaluation of agreement, we found there was very good agreement concerning the presence of intrathecal IgG synthesis (kappa 0.870 to 1.000 between methods, and 0.947 and 0.920 between observers, respectively, representing 0 to 5 out of 114 samples classified differently). The agreement was less pronounced when international consensus classification of isoelectric focusing patterns into 5 different types was take

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Klinická biochemie a metabolismus

ISSN

1210-7921

e-ISSN

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Volume of the periodical

19/40

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

229-233

UT code for WoS article

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EID of the result in the Scopus database

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